Search results for "Microscopic observation"
showing 6 items of 6 documents
Pistachio fruits rust caused by Tuberculina persicina (Ditmar) Sacc., anamorph of Helicobasidium purpureum (Tul.) Pat.
2020
In the study, we found rust in pistachio fruits and verified that it was caused by Tuberculina persicina, anamorph of Helicobasidium purpureum, through isolation and culture, microscopic observation, molecular identification and pathogenicity tests of Koch’s postulates. To our knowledge, this is the first report of rust fruit in pistachio caused by Tuberculina persicina.
Essential Oils as Natural Biocides in Conservation of Cultural Heritage
2020
Essential oils (EOs) have been known for a long time, and they are used in several fields such as medicine and aromatherapy, as well as in the food and pharmaceutical industries. In the last decade, EOs have also been applied to contrast the biodeterioration of cultural heritage, representing a powerful resource in green conservation strategies. In this study, an integrated approach based on microscopic observation, in vitro culture, and molecular investigation was preliminarily employed to identify biological systems colonizing wooden artworks. In order to contrast the biodeterioration processes induced by fungal colonization (Aspergillus flavus) or insect infestation (Anobium punctatum), …
Advances in detection of fastidious bacteria: From microscopic observation to molecular biosensors
2019
Abstract Identification of pathogens and diagnosis of infections are important health challenges, especially in the case of fastidious bacteria which are those difficult-to-grow. A fastidious organism is any organism that has a complex nutritional requirement. Additionally, a fastidious microorganism will only grow when specific nutrients are included in the culture medium. These bacteria can cause serious diseases whose detection and monitoring is critical in many cases. The oldest detection methods are based on simple microscopy observation and staining, after culture on selective growth media, but often do not provide a clear answer. Some new molecular approaches, such as DNA-based seque…
In-Situ atomic force microscopic observation of ion beam bombarded plant cell envelopes
2007
Abstract A program in ion beam bioengineering has been established at Chiang Mai University (CMU), Thailand, and ion beam induced transfer of plasmid DNA molecules into bacterial cells (Escherichia coli) has been demonstrated. However, a good understanding of the fundamental physical processes involved is lacking. In parallel work, onion skin cells have been bombarded with Ar+ ions at energy 25 keV and fluence1–2 × 1015 ions/cm2, revealing the formation of microcrater-like structures on the cell wall that could serve as channels for the transfer of large macromolecules into the cell interior. An in-situ atomic force microscope (AFM) system has been designed and installed in the CMU bio-impl…
Microscopic observation of unworn siloxane-hydrogel soft contact lenses by atomic force microscopy
2006
In the present study, samples of lotrafilcon A, balafilcon A, and galyfilcon A contact lenses were observed by atomic force microscopy (AFM) in tapping mode at areas ranging from 0.25 to 400 m2. Mean roughness (Ra), root-mean-square roughness (Rms) and maximum roughness (Rmax) in nanometers were obtained for the three lens materials at different magnifications. The three contact lenses showed significantly different surface topography. However, roughness values were dependent of the surface area to be analyzed. For a 1 m2 area, statistics revealed a significantly more irregular surface of balafilcon A (Ra = 6.44 nm; Rms = 8.30 nm; Rmax = 96.82 nm) compared with lotrafilcon A (Ra = 2.40 nm; …
Evaluation of efficacy and biocompatibility of a new absorbable synthetic substitute as a dural onlay graft in a large animal model.
2016
Numerous dura substitutes are commercially available, but no absorbable synthetic dura repair product has been used for both onlay and suture applications.The safety and effectiveness of a new absorbable synthetic substitute composed of Poly-L-lactide microfibers as onlay dural graft were evaluated.Physical properties and performance of the microfibrous synthetic dural substitute implanted as an onlay or suturable grafts were compared with these commercial products, including CODMAN ETHISORB™ Dura Patch and DuraGen™ Dural Graft Matrix, in a canine duraplasty model. The cerebrospinal fluid (CSF) leakage, macroscopic and microscopic observation at 30 and 90 days after implantation were invest…